or pvalue. padj stands for the adjusted p values.

ID

B

F

L

S

p

padj

003

747

– 0.35

0.17

– 2.08

0.037

0.163

005

0

NA

NA

NA

NA

NA

419

520

0.21

0.1

2.04

0.041

0.176

457

323

0.02

0.15

0.17

0.866

0.962

460

88

– 0.15

0.26

– 0.57

0.567

0.816

938

0

– 1.73

3.49

– 0.5

0.62

NA

xamine data statistics, the summary function was called. The

this call is shown below. Based on the base two logarithm fold

e., LFC and a critical p value 0.1, the model identified 1,564 up-

genes and 1,193 down-regulated genes. It also shows 39% of

ts and 0.84% outliers within the data set.

y(res.table) #summary of results

of 25258 with nonzero total read count

usted p-value < 0.1

> 0 (up) : 1563, 6.2%

< 0 (down) : 1188, 4.7%

liers [1] : 142, 0.84%

counts [2] : 9971, 39%

an count < 10)

see 'cooksCutoff' argument of ?results

] see 'independentFiltering' argument of

ts

summary function has a parameter alpha, which stands for a

value. Its default value is 0.1. When a critical p value was reduced

to 0.01, the new result is shown below, where the DEG numbers

ced.

ary(res.table,alpha=0.01)

25258 with nonzero total read count

ed p-value < 0.01

0 (up) : 851, 3.4%

0 (down) : 586, 2.3%

rs [1] : 142, 0.56%

unts [2] : 9971, 39%